Enzymes are special molecules that help our bodies do different things, like digest food or fight off germs. Scientists study enzymes to understand how they work and how we can use them to improve our health.

The goal of this experiment is to test the efficiency of lactase enzyme, with varying concentrations and temperatures of lactose.

We're testing the efficiency of lactase enzyme with different concentrations of lactose and temperatures. We'll measure the amount of glucose produced to see how well the enzyme works.


The Efficiency of Lactase


Objectives/Goals:
The goal of this experiment is to test the efficiency of lactase enzyme, with
varying concentrations and temperatures of lactose

Methods/Materials:

The materials used were: Lactose Sugar, Lactase Enzyme, Water bath, Distilled
water, Glucose test strips,10mL test tubes, 600 mL glass bottle, and dextrose.

1) The first test done was to test the glucose test strips
accuracy. The glucose test strips were able to test for concentrations of
glucose at 0.005M, 0.015M, 0.030M, 0.060M, and 0.110M. So, solutions of dextrose, with these molarities, were made. Then they were tested using the glucose test strips. The test showed the strips were accurate.

2) Then 2 litters of a 1M lactose solution was made. 3mL of the solution at room temperature were tested with 2 drops of the lactase enzyme. The glucose test strip was placed in the solution and the concentration was read off of
it.

3) Then 80mL of the solution was heated it up in increments of 5 degrees
starting at room temperature. Every five degrees the solution was tested for glucose. This was continued until the maximum amount of glucose was detected.

4) The lactose solution was then decreased in increments of .2M. At each
concentration, the solution was heated up in increments of 5 degrees and tested
for glucose every 5 degrees.

Results:

When the concentration of the lactose is lowered, the temperature at which the
maximum amount of
glucose is tested becomes lower. So, at lower concentrations of lactose the
lactase becomes more
efficient.

Conclusions:
From the results of this experiment, the researcher can see that as the
concentration is lowered and as the
temperature increases of lactose, more glucose was detected. But, at lower
concentrations, the
temperature wasn't as high when the maximum amount of glucose was detected. More
glucose was
detected as the temperature increases because the bonds in the lactose are
weaker due to the heat. So, the
same amount of lactase will detect more glucose because the enzyme has less work
to do.

Testing the Efficiency of Lactase

The efficiency of lactase

In this demonstration, the effect of lactase on milk, heated milk, and sucrose was investigated.

<h1><strong>1000 Science Fair Project Ideas by Subject</strong></h1><p>Looking for a science fair project idea? Each of our science fair project idea has complete instructions with video. Get ideas for your science projects, find extra resources for your class' scientific projects, or try a fun experiment at home! It doesn't matter what grade you're in, there's something for everyone!</p><p>You can find your perfect science fair project from the different science subjects below or from our <a href="https://www.all-science-fair-projects.com/category0.html#bottom">science fair project idea lists!</a></p>

<h2>Science Fair Project Idea Lists</h2><p>Looking for other ways to find good ideas for science fair projects? Check out these lists of science projects!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>Easy Science Fair Projects</h3><p><img src="/images2/flex/176_popular 2 (2).png" data-size="100" data-float="left" style="width:100px; float:left">Explore the exciting world of science with an easy science fair project that you can do at home using simple materials. Any one of these easy project ideas will teach you important scientific concepts and help you have fun while learning. An easy science fair project is a great way to introduce the wonders of science to kids and adults alike!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><ul><li><p>Changing the Color of Flowers</p></li><li><p>Baking Soda Volcano</p></li><li><p>Swimming Raisins</p></li><li><p>Egg in Vinegar</p></li><li><p>Color of Skittles Experiment</p></li><li><p>Jumping Rice Krispies</p></li><li><p>Mixing Colors of Light</p></li><li><p>Camouflage and Hide</p></li><li><p>Light and Dark on Seed Germination</p></li><li><p>Make a Parachute</p></li></ul><p>More science project details at <a href="https://www.all-science-fair-projects.com/science_fair_project_list/12">Easy Science Fair Projects <strong>→</strong></a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>The Top 10 Best Science Fair Projects</h3><p><img src="/images2/flex/177_popular 2 (3).png" data-size="100" data-float="left" style="width:100px; float:left">To find the best science fair project for you, we've picked the best science projects from 10 different science categories, all with very interesting topics. 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With just a few simple things, you can be fascinated by science and have tons of fun with these popular science projects!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><ul><li><p>Explosive Elephant Toothpaste</p></li><li><p>Egg in Vinegar</p></li><li><p>Electrifying Lemon Battery</p></li><li><p>Making Oobleck: Liquid or Solid?</p></li><li><p>Egg Drop Project</p></li><li><p>Colors of Skittles Experiment</p></li><li><p>Cloud in a Bottle</p></li><li><p>Baking Soda Volcano</p></li><li><p>Egg in a Bottle</p></li><li><p>Plastic Milk</p></li></ul><p>Project details and more cool science projects at <a href="https://www.all-science-fair-projects.com/science_fair_project_list/1/Popular_Science_Experiments_At_Home">Popular Science Fair Projects <strong>→</strong></a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>One-Day Science Fair Project Ideas</h3><p><img src="/images2/flex/172_popular 2 (1).png" data-size="100" data-float="left" style="width:100px; float:left">Quick experiments can be a great option for a science fair project. In this list, we've highlighted one-day science fair project ideas for elementary school (easy), middle school (medium), or high school (hard). These experiments are fast and easy to do, but the science topic packs a punch!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h4>Acid-base reactions: Mixing acids with bases quickly makes carbon dioxide gas!</h4><ul><li><p><a href="https://www.all-science-fair-projects.com/project830.html"><strong>Rocket Film Canisters (easy) </strong></a>Make a rocket fly sky high with just baking soda and vinegar!</p></li><li><p><a href="https://www.all-science-fair-projects.com/project829.html"><strong>Make Your Own PH Indicator (medium) </strong></a>Learn how to make your own acid-base indicator using red cabbage and use it to test different fluids!</p></li><li><p><a href="https://www.all-science-fair-projects.com/project1327.html"><strong>Testing Antacids (hard) </strong></a>Find out which antacid can neutralize the most acid and get relief from heartburn and gastritis!</p></li></ul><h4>Chromatography reactions: Separating out colors as liquid moves through the paper!</h4><ul><li><p><a href="https://www.all-science-fair-projects.com/project826.html"><strong>Colors of Skittles Experiment (easy) </strong></a>Did you know that some Skittles have a secret rainbow hiding inside them?</p></li><li><p><a href="https://www.all-science-fair-projects.com/project678.html"><strong>Uncovering Fall Colors (medium) </strong></a>Have you ever wondered why leaves change color in the fall? 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Biochemistry is the study of the chemistry of living things. Biochemists study how different molecules like DNA and proteins work together to sustain life. The groundbreaking discoveries in biochemistry continue to lead to the discovery of new medicines and cutting-edge treatments for diseases.

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Testing the Efficiency of Lactase

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