Temperature and the Catalase Enzyme
Hard
Let's explore how temperature affects the enzyme Catalase and how it helps convert hydrogen peroxide into oxygen and water. We'll measure the time it takes for a filter paper to float to the top of a test tube to see how temperature affects the enzyme.
Hypothesis
The hypothesis is that at temperatures above 40°C, the enzyme will become less effective.
Method & Materials
You will cut a potato into small pieces, blend it with water, and dip filter paper into the mixture. You will also fill 8 beakers and 8 test tubes with hydrogen peroxide and water, and adjust the temperature of the water in each beaker. Finally, you will place the filter paper into the test tubes and measure the time it takes for the paper to float to the top.
You will need a potato, a blender, filter paper, 8 beakers, 8 test tubes, a hot plate, a packet of ice, a bottle of hydrogen peroxide, a stopwatch, a thermometer, scissors, tweezers, a glass rod, and a marker pen.
Results
The experiment showed that the filter paper floated very soon at temperatures below 40°C. At temperatures above 45°C, the paper took a longer time before floating. This proves that the hypothesis is true, and that at temperatures above 40°C, the enzyme Catalase will become less effective.
Why do this project?
This science project is interesting because it explores how temperature affects the enzyme Catalase, which is found in almost all living organisms and helps to convert hydrogen peroxide into oxygen and water.
Also Consider
Consider repeating the experiment using different amounts of hydrogen peroxide in the test tubes, or using liver tissue instead of potatoes.
Full project details
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