Dissolution Rate of Lactase Pills
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Researched by Baylee B.
2003-04 |
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PURPOSE
The purpose of this experiment was to determine the rate of dissolution
for several dairy digestive supplements, lactase pills.
I became interested in this idea because I am lactose intolerant and
I wanted to buy the fastest acting pill.
The information gained from this experiment would help consumers know
which brand of lactase pills will dissolve fastest so they can make the
best purchasing decision.
HYPOTHESIS
My hypothesis was that the brand from Wal Mart would dissolve faster
than the Western Family brand and Lactase Enzyme brand, and the Lactaid
Ultra brand.
I based my hypothesis on the fact that the Wal Mart brand pill is rougher
than the Western Family brand pill, the Lactase Enzyme, and the Lactaid
Ultra brand pill.
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EXPERIMENT DESIGN
The constants in this study were:
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Amount of water (250ml) for each test
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Temperature of water (37* Celsius)
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Speed of magnetic stirrer (high setting)
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Type of water (tap)
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Amount of pills (one) tested at one time
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Testing procedures
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Type of stopwatch
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Type of pill (lactase)
The manipulated variable was the brand of lactase pills.
The responding variable was the dissolution time.
To measure the responding variable I will use a stopwatch accurate to
1 one hundredth of a second.
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MATERIALS
Quantity |
Item Description |
5 |
lactase pills for each of the 4 brands |
2 |
magnetic stirrer |
600ml |
of tap water |
2 |
300 ml glass beaker |
1 |
celsius thermometer |
1 |
pair of heat resistant |
1 |
thick lap apron |
1 set |
goggles |
1 |
microwave |
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PROCEDURES
1. Measure 250ml of tap water
2. Pour 250ml of water into a microwave safe container
3. Heat water in microwave to 37* Celsius
4. Pour heated water into 300 ml glass beaker (Only up to the 250 mark
though)
5. Put onto magnetic stirrers base
6. Drop in magnetic stirring capsule
7. Turn on magnetic stirrer to high (Let the stirrer get going fast
before you drop in the pill)
8. Drop in first brand of lactase pill
9. Immediately Start stopwatch
10. When the pill dissolves (disappears) stop the stop watch then the
stirrer
11. Record the dissolution time of the pill in minutes and seconds
12. Repeat steps 1-11 for the other pills of this same brand (5 more
times)
13. Repeat steps 1-12 using the next brand of pills
14. Repeat steps 1-13 with the remaining brands
15. Average the dissolution rate for each brand.
16. Compare brands
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RESULTS
The original purpose of this experiment was to determine which brand
of lactase pills would dissolve the fastest.
The results of the experiment were that the Lactase Enzyme brand dissolved
the fastest.
See my data table
and graph.
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CONCLUSION
My hypothesis was that the brand Equate brand would dissolve faster
than the Western Family brand and Lactase Enzyme brand, and the Lactaid
Ultra brand.
The results indicate that this hypothesis should be rejected.
Because of the results of this experiment, I wonder if I used a mixture
of simulated stomach acid (hydrochloric).
If I were to conduct this project again I would have tried to get some
more brands of lactase pills so that the experiment would have been more
meaningful.
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RESEARCH REPORT
Dissolution
Dissolution is the act or process of breaking up into parts; disintegration.
A lactase pill can use the act of dissolution because it will break up
into tiny particles not just stay all together.
Solvent/Soluble
A Solvent is a liquid that is capable of dissolving another substance.
Something that is a solvent is water. Water is a solvent because it can
dissolve things like lactase pills. Many other liquids are solvents as
well. Soluble means capable of being dissolved. Soluble things are also
called solutes. A solute is like a lactase pill. A lactase pill is a solute
because it is capable of being dissolved. Aspirin is also a solute because
it can be dissolved.
Rate
Rate is a measured quantity that occurs or is attained within the limits
of a fixed quantity. In this project you would use rate to write down the
times that the lactase pills dissolved.
Milk
The milk of cows is used as food by humans. Milk is used in many baked
goods and other things that you can cook or bake. Yogurt, butter, cheese,
cottage cheese, cream, sour cream, and liquid milk all are milk products,
so they all contain some lactose. Butter is churned milk fat. Cheese is
treated curd of milk. Cream is rich milk containing 18% milk fat. Ice cream
is frozen dairy products. Yogurt is thick custard like form of cultured
milk.
Milk is considered a very healthy food and part of a well balanced diet.
A person is supposed to have four to six servings of dairy a day. Without
being able to have milk then you wouldnt be able to stay healthy because
you wouldnt have your four to six servings a day. That is why it is very
important to have your full four to six servings a day. -
Milk has a lot of different ingredients. The composition of milk is:
* 87. 4% Water
*. 7% Minerals
* 3. 5% Fat
* 3. 5% Protein
* 4. 9% Carbohydrates (including lactose)
One ingredient is lactose, which makes up about 5%. Anything with milk
in it has at least a little bit of lactose inside of it. Some people become
ill when they eat lactose containing foods. Without lactase pills
lactose intolerant people wouldnt be able drink milk or eat any other
milk products. That would cut out a lot of the very healthy foods from
their diets. It wouldnt be healthy to eliminate all milk products from
ones diet. That is why Lactase is very important.
Lactose
Lactose is a white crystalline sugar that is found in milk and has
the formula C12H22O11. It is used in infant foods, bakery, and various
sweets. Lactose intolerence is caused by genetic metabolic defects where
certain enzymes are missing. Lactose intolerance usually affects Asian,
African Americans, and Indians. Lactose intolerance is a life long condition.
70% of the worlds population is lactose intolerance. 1 to 3% of babies
are lactose intolerance. The difference between lactose and lactase is
that lactase is the enzyme that breaks up lactose. Lactose intolerance
can make you have very bad stomach aches.
Lactase
Lactase is an enzyme found in some yeasts and in the digestive juices
of mammals. That decomposes lactose into simpler sugars like glucose. Lactase
breaks down the sugar in milk called Lactose. Lactase helps people with
lactose intolerance because without lactase the lactose would hurt our
stomachs. Without Lactose then there would be no reason for lactase.
Enzyme
An Enzyme is a molecule that speeds up chemical reactions in
all living things. No life would be possible without enzymes. Reactions
would occur to slowly or not at all without enzymes. Life would not be
possible without enzymes. Every living cell makes an enzyme. Enzymes are
not alive. Altered molecules combined with enzymes forms a complex molecular
structure in which chemical reactions take place. The enzyme would then
separate from the product of the reaction. Most enzymes are proteins. A
small number or ribonucleic acid molecules also function as enzymes. The
human body has thousands of different kinds of enzymes. Each different
brand has its own use. Without enzymes a person could not move, digest
food, see, or breathe. Most enzymes break down complex substances into
simpler ones. The word enzyme comes from two Greek words meaning in yeast.
Scientists who studied in the 1800s studied reactions caused by yeast
enzymes. James B. Sumner was the first person to isolate a pure enzyme
in the form of crystals.
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BIBLIOGRAPHY
Barry, Roger D. Lactic Acid. World Book Encyclopedia, 1999.
Dissolving. Young Scientist pp. 19?21.
Lactose. Websters Family Encyclopedia, 1990, 6.
Marshall, Robert T. "Milk. World Book Encyclopedia, 1998.
Mayo Clinic Family Health Book. New York, New York, William Morrow
and Company Pgs. 772, 1049.
The New Good Housekeeping Family Health and Medical Guide. New York,
New York, Hearst Cooperation 1989, pgs. 497.
Rudolph, Fredrick B. , Enzyme World Book Encyclopedia, 1998.
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ACKNOWLEDGEMENTS
I would like to thank the following people for helping make my project
possible:
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My mom for buying me the lactase pills that I needed
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Mr. Newkirk for encoring me to keep working
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Mrs. Helms for telling me to keep working
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