Virology is the study of viruses, tiny particles that can make us sick. By studying virology, we can learn how to prevent and treat viral diseases, like the flu and COVID-19.

Phase variation in the colonial opacity of Streptococcus pneumoniae has been implicated as a factor in bacterial adherence, colonization, and invasion in the pathogenesis of pneumococcal disease. Additionally, the synergistic effects of influenza A virus and S. pneumoniae in the development of otitis media (OM) have been reported. This study examined the ability of opaque or transparent S. pneumoniae from the same strain in combination with an antecedent influenza A virus infection to colonize the nasopharynx and invade the middle ear in the chinchilla model.

In this project, we will explore the effects of the Influenza A Virus on the colonization of the nasopharynx and otitis media in the chinchilla model. We will investigate how the virus affects the adherence, the kinetics of colonization, and invasion of the middle ear by isogenic opaque and transparent variants of S. pneumoniae type 6A.

 
EFFECT OF INFLUENZA A VIRUS INFECTION ON NASOPHARYNGEAL COLONIZATION AND OTITIS MEDIA INDUCED BY TRANSPARENT OR OPAQUE PHENOTYPE VARIANTS OF STREPTOCOCCUS
PNEUMONIAE IN THE CHINCHILLA MODEL

BACKGROUND:

Otitis media (OM) is one of the most common childhood diseases. The prevalence, medical care costs, and hearing-related morbidity of OM are significant. Streptococcus pneumoniae, the primary etiological agent, has been isolated from approximately 20 to 50% of middle-ear effusions from children with OM (4). The process whereby S. pneumoniae becomes established in the human nasopharynx and then affects the transition from a colonized to a diseased state in the middle ear is not yet known.

Many children with OM experience an antecedent viral upper-respiratory-tract infection, and viruses are also recognized as important etiological agents of OM, either alone or in combination with bacterial pathogens. Influenza A virus, adenovirus, and respiratory syncytial virus are the primary respiratory-tract viruses associated with this disease (5, 6). A recent study with adult human volunteers indicates that influenza A virus infection promotes significant colonization of the nasopharynx by S. pneumoniae (21).

GOAL:

This study was designed to further assess the effects of influenza A virus on adherence, the kinetics of colonization, and invasion of the middle ear by isogenic opaque and transparent variants of S. pneumoniae type 6A in the chinchilla model of OM.

PROCEDURE:

A total of 48 healthy chinchillas (Chinchilla lanigera) (220 to 450 g) free of middle-ear disease, as determined by otoscopyand tympanometry, were used in these studies. Two cohorts of 12 chinchillas each were inoculated intranasally (i.n.) with influenza A virus followed 7 days later by i.n. inoculation with the S. pneumoniae type 6A opaque or transparent variant. Another two cohorts of 12 chinchillas each were inoculated i.n. with diluent only (without influenza A virus) followed 7 days later by i.n. inoculation of either opaque or transparent S. pneumoniae; these cohorts served as controls. Three chinchillas (preselected and randomized) from each cohort were evaluated for NP colonization and OM at each of four different time periods after S. pneumoniae type 6A inoculation as described below. The ability of influenza A virus to promote NP colonization by S. pneumoniae and development of OM was compared for the opaque and transparent variants. All experiments were performed in duplicate.

CONCLUSION:

The results from the present study indicate that the effects of influenza A virus on the pathogenesis of experimentalS. pneumoniae-induced OM vary depending on the opacity phenotype of the S. pneumoniae inoculum.


The Effects of Influenza A Virus

What is the effect of the Influenza A Virus Infection on Nasopharyngeal Colonization?

<h1><strong>1000 Science Fair Project Ideas by Subject</strong></h1><p>Looking for a science fair project idea? Each of our science fair project idea has complete instructions with video. Get ideas for your science projects, find extra resources for your class' scientific projects, or try a fun experiment at home! It doesn't matter what grade you're in, there's something for everyone!</p><p>You can find your perfect science fair project from the different science subjects below or from our <a href="https://www.all-science-fair-projects.com/category0.html#bottom">science fair project idea lists!</a></p>

<h2>Science Fair Project Idea Lists</h2><p>Looking for other ways to find good ideas for science fair projects? Check out these lists of science projects!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>Easy Science Fair Projects</h3><p><img src="/images2/flex/176_popular 2 (2).png" data-size="100" data-float="left" style="width:100px; float:left">Explore the exciting world of science with an easy science fair project that you can do at home using simple materials. Any one of these easy project ideas will teach you important scientific concepts and help you have fun while learning. An easy science fair project is a great way to introduce the wonders of science to kids and adults alike!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><ul><li><p>Changing the Color of Flowers</p></li><li><p>Baking Soda Volcano</p></li><li><p>Swimming Raisins</p></li><li><p>Egg in Vinegar</p></li><li><p>Color of Skittles Experiment</p></li><li><p>Jumping Rice Krispies</p></li><li><p>Mixing Colors of Light</p></li><li><p>Camouflage and Hide</p></li><li><p>Light and Dark on Seed Germination</p></li><li><p>Make a Parachute</p></li></ul><p>More science project details at <a href="https://www.all-science-fair-projects.com/science_fair_project_list/12">Easy Science Fair Projects <strong>→</strong></a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>The Top 10 Best Science Fair Projects</h3><p><img src="/images2/flex/177_popular 2 (3).png" data-size="100" data-float="left" style="width:100px; float:left">To find the best science fair project for you, we've picked the best science projects from 10 different science categories, all with very interesting topics. 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With just a few simple things, you can be fascinated by science and have tons of fun with these popular science projects!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><ul><li><p>Explosive Elephant Toothpaste</p></li><li><p>Egg in Vinegar</p></li><li><p>Electrifying Lemon Battery</p></li><li><p>Making Oobleck: Liquid or Solid?</p></li><li><p>Egg Drop Project</p></li><li><p>Colors of Skittles Experiment</p></li><li><p>Cloud in a Bottle</p></li><li><p>Baking Soda Volcano</p></li><li><p>Egg in a Bottle</p></li><li><p>Plastic Milk</p></li></ul><p>Project details and more cool science projects at <a href="https://www.all-science-fair-projects.com/science_fair_project_list/1/Popular_Science_Experiments_At_Home">Popular Science Fair Projects <strong>→</strong></a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>One-Day Science Fair Project Ideas</h3><p><img src="/images2/flex/172_popular 2 (1).png" data-size="100" data-float="left" style="width:100px; float:left">Quick experiments can be a great option for a science fair project. In this list, we've highlighted one-day science fair project ideas for elementary school (easy), middle school (medium), or high school (hard). These experiments are fast and easy to do, but the science topic packs a punch!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h4>Acid-base reactions: Mixing acids with bases quickly makes carbon dioxide gas!</h4><ul><li><p><a href="https://www.all-science-fair-projects.com/project830.html"><strong>Rocket Film Canisters (easy) </strong></a>Make a rocket fly sky high with just baking soda and vinegar!</p></li><li><p><a href="https://www.all-science-fair-projects.com/project829.html"><strong>Make Your Own PH Indicator (medium) </strong></a>Learn how to make your own acid-base indicator using red cabbage and use it to test different fluids!</p></li><li><p><a href="https://www.all-science-fair-projects.com/project1327.html"><strong>Testing Antacids (hard) </strong></a>Find out which antacid can neutralize the most acid and get relief from heartburn and gastritis!</p></li></ul><h4>Chromatography reactions: Separating out colors as liquid moves through the paper!</h4><ul><li><p><a href="https://www.all-science-fair-projects.com/project826.html"><strong>Colors of Skittles Experiment (easy) </strong></a>Did you know that some Skittles have a secret rainbow hiding inside them?</p></li><li><p><a href="https://www.all-science-fair-projects.com/project678.html"><strong>Uncovering Fall Colors (medium) </strong></a>Have you ever wondered why leaves change color in the fall? 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This is how we can discover the answer to our question!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><ul><li><p>What makes a cloud form?</p></li><li><p>Can drink and food taste different just by changing its color?</p></li><li><p>Does the color of light affect photosynthesis?</p></li><li><p>Does temperature affect seed sprouting?</p></li><li><p>What makes popcorn pop?</p></li></ul><p>More <a href="https://www.all-science-fair-projects.com/science_fair_project_list/14">testable questions with their science projects <strong>→</strong></a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h2>Science Fair Project FAQ</h2><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>What are some hands-on ways to find inspiration for my science fair project?</h3><h4>Science museums</h4><p><img src="/images2/flex/124_7.png" data-size="100" data-float="left" style="width:100px; float:left">Science museums are great places to explore if you're looking for inspiration for your next science fair project or just want to learn more about science. Science museums, natural history museums, space museums, and discovery museums are all great places to do that! You can discover all sorts of interesting things about science and find cool project ideas for science fairs.</p><p>There may be free admission days or free passes to a science museum near you! Check your credit card for offers, your local library for free museum passes, and nearby science museums for free entrance days.</p><p>So what are you waiting for? <a href="https://en.wikipedia.org/wiki/List_of_science_museums">Find a science museum near you</a> and prepare to be awed by all that you can learn there!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h4>Look around and observe</h4><p><img src="/images2/flex/117_5.png" data-size="100" data-float="left" style="width:100px; float:left">Do you know how scientists start their cool projects? They often look around and notice things that are interesting or make them curious. Then they start doing an experiment! You can do the same! Look around your school, home, or community. Is there anything that catches your attention and makes you curious? Maybe you notice something interesting or a problem that people are facing. This could be a great starting point for your science fair project!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h4>Try an Easy Science Project!</h4><p><img src="/images2/flex/144_easy square.png" data-size="100" data-float="left" style="width:100px; float:left">These science projects are easy and fun to do. You may discover a simple experiment that sparks your interest and inspires you to do more experiments, turning it into a science fair project! Check them out at <a href="https://www.all-science-fair-projects.com/science_fair_project_list/12">Easy Science Fair Projects <strong>→</strong></a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3 style="text-align: start"><strong>How do I start a science fair project?</strong></h3><p><img src="/images2/flex/199_science coach.png" data-size="100" data-float="left" style="width:100px; float:left">No matter if you're in high school, elementary or middle school, science fair projects are a fun and unique way to discover science by asking a question and doing a science project. We'll help you find a science fair project idea and guide you through the process of doing and presenting your science fair project with <a href="https://www.all-science-fair-projects.com/science_fair_projects_coach/science-project-coach.html">Science Fair Coach's Science Fair Project Guide</a>.</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>What should I do after I have a science fair project idea?</h3><p><img src="/images2/flex/143_intro science fair.png" data-size="100" data-float="left" style="width:100px; float:left">If you have a science fair project idea, Science Fair Coach can help you turn it into a full science fair project! Starting at the <a href="https://www.all-science-fair-projects.com/pgpage/4">Introduction to Science Fairs</a>, check out the section on <strong>How to do a science fair project</strong> for a step-by-step guide!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><p><img src="/images2/flex/203_compared to scientific method.png" data-size="100" data-float="left" style="width:100px; float:left">We'll guide you through every step of the <a href="https://www.all-science-fair-projects.com/pgpage/5">scientific method</a>, from designing and conducting your experiment to collecting and analyzing your findings.</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><p><img src="/images2/flex/202_engineering design process.png" data-size="100" data-float="left" style="width:100px; float:left">If you're working on an engineering project, you can head over to <a href="https://www.all-science-fair-projects.com/pgpage/9">The Guide to the Engineering Design Process</a>.</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><p> <img src="/images2/flex/150_science fair poster.png" data-size="100" data-float="left" style="width:100px; float:left">Finally, we'll help you showcase your results on a <a href="https://www.all-science-fair-projects.com/pgpage/7">science fair board</a> for others to learn from and enjoy.</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3 style="text-align: start"><strong>How do I make a science fair board?</strong></h3><p style="text-align: start"><img src="/images2/flex/150_science fair poster.png" data-size="100" data-float="left" style="width:100px; float:left">Your science fair board is where you show off your science fair project for everyone to see and learn from. With <a href="https://www.all-science-fair-projects.com/science_fair_projects_coach/science-project-display-board-6a.html">Science Fair Coach's step-by-step guide</a>, you can make sure your science fair board stands out and impresses at the science fair!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>Where can I find a science fair competition?</h3><p><img src="/images2/flex/179_Intro Science Fair Projects 32.png" data-size="100" data-float="left" style="width:100px; float:left">Science fair competitions are an excellent opportunity to explore science. One of the of the premier science advocacy organizations, the Society for Science, is associated with more than 400 science fair competitions in almost every US state, as well as over 70 other countries, regions, and territories. You can find out if there's a <a href="https://findafair.societyforscience.org/">Society of Science affiliated science fair</a> near you!</p><p>The <a href="http://physics.usc.edu/~gould/ScienceFairs/">The www Virtual Library: Science Fairs website</a> also has a collection of science fairs from all over the world, as well as national, state, regional, local, and virtual competitions!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat">

Microbiology is the study of tiny living things that we can't see with our eyes, like bacteria, viruses and fungi. Microbiologists study how these microorganisms grow, reproduce, and interact with their environment, and how they can affect our health.

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The Effects of Influenza A Virus

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