Testing Toxicity with Lettuce Seeds
Hard
Are you curious about how different concentrations of salt can affect lettuce seeds? Let's find out by conducting a reference toxicity test. We'll mix up different concentrations of salt and then measure the response of the lettuce seeds.
Hypothesis
The hypothesis is that the lettuce seeds will respond in a predictable manner to varying concentrations of salt.
Method & Materials
You will treat the lettuce seeds in a bleach solution, label a series of beakers with different concentrations of salt, add the appropriate test solution to each petri dish, add 5 lettuce seeds to each dish, place the dishes in a plastic bag, incubate the seeds in the dark for 5 days, and then count how many seeds have germinated and measure the root length of each.
You will need lettuce seeds, deionized or distilled water, a 0.2M NaCl solution, 9-cm petri dishes, 7.5-cm paper filter, bleach, and a plastic bag.
Results
After conducting the experiment, we can conclude that the lettuce seeds responded in a predictable manner to varying concentrations of salt. The most interesting observation was that at some threshold concentration, all of the test organisms were killed.
Why do this project?
This science project is interesting because it demonstrates how a simple organism can be used to test the toxicity of a compound.
Also Consider
Experiment variations to consider include testing different compounds and using different concentrations of the same compound.
Full project details
You can find additional information and details for this science fair project here. Have fun exploring!Related video
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