The hypothesis is that repeated use of antibiotics causes bacteria to develop resistance which in turn, causes the antibiotic to become less effective in eliminating the bacteria.

You will prepare 15 Petri dishes, mix different concentrations of antibiotics, and inoculate the Petri dishes with bacteria. After 4 days, you will measure the diameter of the inhibition zone and record your results.

You will need 15 agar Petri dishes, 15 disinfected swabs, 1 bottle of disinfected water, 1 piece of filter paper, 1 hole puncher, 4 test tubes, 1 measuring cylinder, 100mg of antibiotic ampicillin, 1 digital weighing scale, 1 beaker of water, E Coil bacteria culture, 1 forceps, and 1 marker pen.

The results of this experiment show that with each generation, the inhibition zone grows smaller in diameter. Also, the higher the concentration of the antibiotic used, the bigger the diameter of the inhibition zone. This proves that the hypothesis is true and that the use of antibiotics should be avoided unless necessary.

This science project is interesting and unique because it explores how bacteria can become resistant to antibiotics and how this affects our health. It also shows how the excessive use of antibiotics can lead to a longer recovery time for patients who are suffering from the infection.

Variations of this experiment include varying the types of bacteria and antibiotics, and varying the temperature at which the bacteria are left to incubate.

Hey there! Here are some awesome videos about this science project that we think you'll really like. They're not only super fun, but they'll also help you learn more about the science behind the project. So sit back, relax, and get ready to have some fun!!