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Topics  > Biochemistry  > Plant Hormones

Project title What effect does growth hormone supplementation have on irradiated lima beans.
Difficulty Level High school
From the web? Yes
Main link Click here to view all details of this exciting project (Cached)

Description
What effect does growth hormone supplementation have on irradiated lima beans. 

Ingredients
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Forty-eight lima beans were irradiated with high-energy X-rays at Providence St. Joseph's hospital. Sixteen beans were irradiated at 10,000 rads, sixteen beans at 30,000 rads, and sixteen beans at 60,000 rads. In addition, sixteen beans were not irradiated and served as controls. Following irradiation, the lima beans were placed in a hydroponic germination station, where they were exposed to artificial light 16 hours of every 24-hour period, for nine consecutive days. Half of the beans were given growth hormone supplementation (Hormex Growth Hormone) and half were hydrated with clean water only. After nine days, the plants were uprooted and the stem and root lengths were measured. 

Brief Instructions
Lima bean growth was severely stunted at radiation levels of 30,000 and 60,000 rads, since the beans that were irradiated at these levels germinated and then stopped growing altogether. When we compared the data taken from the plants supplemented with growth hormone, the hormone-treated plants generally had longer roots, yet shorted stems. Strangely, at 60,000 rads, there were a few centimeters of stem growth on most plants, yet no visible root growth. 


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